Department of Proteomics

We aim to understand the molecular mechanisms regulating the normal and pathological hematopoiesis as well as those mediating the effect of therapeutical procedures. We complete the basic techniques of cell biochemistry, molecular biology and proteome analysis (gel electrophoresis, western-blotting, flow-cytometry, immunofluorescence microscopy, preparation and transfection of plasmids for exogenous expression of proteins fused to various tags) by other methods and approaches to get a comprehensive view of the protein behavior.

Besides performing basic and applied research, we also assist in introduction of novel diagnostic methods for hematology.

• We participated in the Independent Validation Initiative at antibodies-online and validated the Nucleophosmin antibody. See the results.

Main current topics

• Mechanisms of chemotherapy-induced changes in apoptosis-related proteins
• Consequences of C-terminal nucleophosmin mutations in acute myeloid leukemia
• Mechanisms of immune escape in acute myeloid leukemia (AML)
• Resistance to therapy in chronic myeloid leukemia - novel markers and possible treatment targets
• Chronic myeloid leukemia-derived models of resistance to therapy – development and characterization
• Exosomes, proteomics, and cell surface markers in CML
• Screening of therapeutic targets in chronic myeloid leukemia cells resistant to general treatment
• Interaction of hematopoietic cells with the extracellular matrix
• Regulation of cell adhesion signaling in B-ALL immunotherapy