Department of Cellular Chimerism

The workplace is incorporated into the Complement of Laboratories at ÚHKT. It continuously defends an independent and impartial assessment of the professional competence of the Complement of Laboratories according to the standard ČSN EN ISO 15189, whose application is verified by the Czech Institute for Accreditation. Examination methods within the scope of accreditation are defined by the annex to the Accreditation Certificate in its current version, which is available on the ÚHKT website.

The primary focus of the department is the examination of cellular chimerism through genotyping and quantification of variable polymorphisms such as Short Tandem Repeats (using PCR method and subsequent fragment analysis of PCR products on a genetic analyzer) and short insertions and deletions (using quantitative real-time PCR). The examination is initially performed in patients indicated for allogeneic hematopoietic stem cell transplantation and their donors. Subsequently, long-term monitoring of the post-transplant course occurs in patients, with chimerism analysis helping to determine the dynamics of graft engraftment and monitoring any potential return of the patient's hematopoiesis, which may indicate a risk of disease relapse. With an increase in chimerism in patients after haploidentical transplantation, it is possible to determine whether there has been a loss of HLA-mismatched haplotype in the patient's leukemic cells. The laboratory also analyzes peripheral blood samples from patients after organ transplantation, where it determines the proportion of the organ donor's hematopoiesis. 

The Department of Cellular Chimerism is a reference laboratory (National Reference Laboratory for DNA Diagnostics). It organizes external quality assurance comparisons and offers consulting services. 

Employees are bound by confidentiality under the Act on Health Services, 372/2011 Coll.

Trust and high data security is one of the fundamental priorities in the activities of the Complement of Laboratories at ÚHKT. The established procedures are carried out in accordance with the Personal Data Protection Act 110/2019 Coll., and Regulation (EU) 2016/679 of the European Parliament and of the Council of 27 April 2016 on the protection of natural persons with regard to the processing of personal data and on the free movement of such data and repealing Directive 95/46/EC. More information is available on the ÚHKT website.

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