Laboratory of HLA Serology

The Laboratory of HLA Serology is engaged in:

  • serotyping of HLA antigens (Human Leukocyte Antigens = a major histocompatibility complex)
  • screening and identification of HLA antibodies
  • provision of lymphocytotoxic crossmatch tests

The basic serological method used for all the mentioned examinations is the standard microlymphocytotoxic test.

Serotyping of HLA class I antigens is performed chiefly:

  • with specialized donors of blood and blood components, especially thrombocytes destined to refractory patients
  • in patients  with thrombocytes transfusion refractoriness
  • within differential diagnostics of some diseases in the field of rheumatology, immunology, ophthalmology and others; individual HLA antigens are also identified (mainly HLA-B27)

Screening and identification of HLA antibodies is performed:

  • in the framework of complex prevention of allosensitization to HLA in polytransfunded patients, especially in potential candidates to hematopoietic stem cells transplantation
  • in the selection of HLA-matched donor for patients already refractory to thrombocytes  products administration
  • in the prevention of TRALI (Transfusion Related Acute Lung Injury), a serious post-transfusion complication, in blood donors who had immunization  stimuli (transfusion, pregnancy) in their anamnesis

Lymphocytotoxic crossmatch is performed:

  • in the selection of thrombocyte donors for refractory or potentially refractory patients
  • before the transplantation of hematopoietic stem cells from HLA-haploidentical donors

The laboratory staff members are involved in teaching activity in the framework of  post-graduate education of physicians and non-physicians with a university degree.

The Laboratory of HLA Serology is accredited by the Czech Accreditation Institute (CAI) according to the Czech standard ČSN EN ISO 15189:2007.

Quick contact

Head of the Lab Complex
Tel:+420 221 977 208
Fax:+420 221 977 208

Deputy Head of the Lab Complex
Tel:+420 221 977 263

Opening hours

Mo – Fr: 7:00–18:00
Weekends: 9:00–13:00

Mo – Fr: 7:00–10:30

Inpatient department
Mo – Fr: 13:00–18:00

ICU and Transplant unit
Mo – Fr: 14:00–17:00

How to find us


Ústav hematologie a krevní transfuze
(​Institute of Hematology and Blood Transfusion)

U Nemocnice 2094/1
128 00 Praha 2

The nearest underground station: Karlovo náměstí (line B)

The nearest tram station: Karlovo náměstí (10, 16, 22), Moráň (3, 6, 10, 16, 18, 24)

The nearest bus station: U Nemocnice (148), Karlovo náměstí (176)

How to get here