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NRL for DNA Diagnostics

The main areas of the NRL include molecular-genetic diagnostics, monitoring of oncohematologic diseases and HLA typing for transplantation of hematopoietic stem cells.

For these purposes, the NRL provides reference samples and material and evaluates results of other laboratories in accordance with international standards.

The laboratory also provides consultant services and organizes conferences, seminars and workshops.

Our working groups organize external proficiency testing (EPT) for several examinations. The NRL also participates in a wide range of research projects and educational activities.

Since 2002, the NRL for DNA Diagnostics has been accredited by the Czech Institute for Accreditation as a testing laboratory number 1345 (according to the Czech version of the European Standard EN ISO/IEC 17025 Conformity assessment – General requirements for the competence of testing and calibration laboratories) for molecular genetic analysis of human genome using PCR methods (see the list below).

In 2004, the NRL for DNA Diagnostics was accredited by the Czech Institute for Accreditation as the first medical laboratory number M8001 according to CSN EN ISO 15189. Since November 1st 2015 all examinations have been provided as a part of the IHBT Lab Complex and the activity of the NRL as an accredited medical laboratory was finished.

Form for Laboratory Assessment (only in Czech).


Working groups and organized determinations:

Department of Molecular Genetics

Head of the Department: Mgr. Machová Poláková Kateřina, Ph.D.

Deputy Head of the Department: Mgr. Hana Žižková, Ph.D.

Accredited determinations:

  • Determination of BCR-ABL fusion gene rearrangements using multiplex RT PCR
    (NRL_03_SOP_14_02)
  • Determination of BCR-ABL transcript level using real-time RT PCR
    (NRL_04_SOP_14_02/procedure A)
  • Determination of BCR-ABL kinase domain mutation using direct sequencing
    (NRL_04_SOP_14_02/procedure B)
  • Determination of the conversion factor for expressing the results of BCR-ABL transcript level in international scale
    (NRL_04_SOP_14_02/procedure C)

Department of HLA

Head of the Department: Ing. Milena Vrana

Cell chimerism group

Technical head: Mgr. Hana Čechová

Genotyping of sequence polymorphisms used to identify of cell population (procedure A) and optionaly quantitative representation of cell population in the sample is followed (procedure B). Polymorphisms of short tandem repeal (STR), single nukleotide polymorphisms (SNP) and short insertion and deletion (indels) are determined by fragment analysis or quantitative real-time PCR.

Cell chimerism group annually internationally organize the External Proficincy Testing  for Quantitative Analysis of Cell Chimerism after Allogeneic HSCT.

Accredited determinations:

  • Genotyping of sequence polymorphisms by electrophoresis using commercial kits
    (NRL_01_SOP_14_02/ procedure A)
  • The assessment of quantitative representation of genotypes in the sample of sequence polymorphisms by electrophoresis using commercial kits
    (NRL_01_SOP_14_02, procedure B)
  • Genotyping of sequence polymorphisms by real-time PCR
    (NRL_07_SOP_14_02, procedure A)
  • The assessment of quantitative representation of genotypes in the sample of sequence polymorphisms by real-time PCR
    (NRL_07_SOP_14_02, procedure B)

Quick contact

Head of the Lab Complex
Tel:+420 221 977 208
Fax:+420 221 977 208

Deputy Head of the Lab Complex
Tel:+420 221 977 263


Opening hours

Ambulance
Mo – Fr: 7:00–18:00
Weekends: 9:00–13:00

Donors
Mo – Fr: 7:00–10:30

Visitors
Inpatient department
Mo – Fr: 13:00–18:00

ICU and Transplant unit
Mo – Fr: 14:00–17:00

How to find us

map

Ústav hematologie a krevní transfuze
(​Institute of Hematology and Blood Transfusion)

U Nemocnice 2094/1
128 20 Praha 2

The nearest underground station: Karlovo náměstí (line B)

The nearest tram station: Karlovo náměstí (10, 16, 22), Moráň (3, 6, 10, 16, 18, 24)

The nearest bus station: U nemocnice (291), Karlovo náměstí (176)

How to get here